M. Khandaker, S. Mesiya, R. Wolf, M.B. Vaughan
University of Central Oklahoma,
United States
Keywords: deep wound healing, skin substitute, polymer, nanofiber, antimicrobial agents
Summary:
Introduction: Prolonged antimicrobial and osteoinductive activities of polycaprolactone (PCL) nanofiber membrane (NFM) for biomedical application is possible by tethering the antimicrobial and osteoinductive molecules with PCL NFM [1]. The goal of this study to evaluate the in vitro antimicrobial properties of PCL with and with MgO nanoparticles using Staphylococcus aureus (ATCC 6538) and in vivo biocompatibility of MgO-PCL using rat skin wound dressing model. Materials and Methods: The antibacterial activity was estimated using growth kinetic models, disk diffusion tests, and microbial penetration test using standard protocols [2-4]. The bacteria suspension was diluted to the concentration of 10^8 CFUs/mL and spread onto an infusion agar plate. All circular samples were sterilized and then gently pasted on the inoculated plates. After incubation at 37 °C for 24 h, the diameters of inhibition zones around the discs were measured using a Vernier calliper. All experiments were performed in triplicate. To investigate the films’ ability to inhibit microbial penetration, each film was tightly placed on an open test tube containing 5 mL autoclaved nutrient broth (NB) medium. The transmittance of NB was measured at 600 nm as an indicator of microbial contamination. This study used Lewis breed inbred rat to see whether PCL membrane can serve as an antibacterial protective membrane. Results: The antibacterial activity was improved due to the incorporation of MgO nanoparticles. PCL MgO nanofibers membrane exhibited significantly stronger antibacterial activities against S. aureus compared to PCL nanofiber membrane. The in vivo study shows that there is no adverse effect (inflammation) of rat due to the application of the membrane application. Discussion: The results have significant implications for finding a safe and an inexpensive path, especially led us to a conclusion that we could use the fiber material for both in vitro and in vivo applications. This study found the appropriate concentration of MgO with PCL that shows no lethal effects on fibroblast cells and show defiantly lethal effect against the bacterial growth. Significance: A facile strategy to incorporate immobilizing MgO (Magnesium Oxide) Nanoparticles with Polycaprolactone nanofiber matrix (PCL) supported by acrylic molds and to detect their antibacterial activity is proposed for emerging antibacterial bandage applications. The antibacterial activity was evaluated against S. aureus. The in vivo efficacy of polycaprolactone nanofiber membrane (PCL NFM) using rat skin model shows potential clinical application of our bandage material.