FluidFM in Live Cell Biology: From Biomechanics to Single-Cell Omics

O. Guillaume-Gentil

Keywords: FluidFM, single-cell, omics


As single-cell analysis continues to transform biomedical research, there is a growing demand for technologies enabling non-destructive manipulation and analysis of living cells. Fluidic Force Microscopy (FluidFM) (1) integrates microfluidics, atomic force microscopy, and optical microscopy into a microscopic pipette tailored to the scale of single cells. With its subcellular precision and the ability to handle volumes in the pico to femtoliter range, FluidFM facilitates selective perturbation and analysis of individual cells within physiological environments while preserving their viability. Over the years, we've expanded the technology for various complementary applications, ranging from biomechanical measurements to the cellular injection and extraction of biomolecules and organelles (2). Most recently, we introduced Live-seq (3), a cutting-edge single-cell transcriptomics approach that allows for spatial and temporal analysis of single-cell gene expression and phenotypic changes. Our ongoing research leverages these methodologies to explore cellular dynamics and cell-cell communication during viral infection and stem cell differentiation. References: (1) Meister, Gabi et al., Nano Letters 2009, DOI: 10.1021/nl901384x (2) Guillaume-Gentil et al., Trends Biotech 2014, DOI: 10.1016/j.tibtech.2014.04.008 (3) Chen, Guillaume-Gentil et al., Nature 2022, DOI: 10.1038/s41586-022-05046-9