National Institute of Standards and Technology,
Keywords: DNA nanotechnology, self-assembly, multivalency, nanofabrication
Summary:Nanoscale spatial organization of bioactive components has the potential to open exciting possibilities in influencing cellular processes. Whether in the localization of numerous antibodies in phagocytosis of viruses, the clustering of proteins in supramolecular organizing centers (SMOCs), or the adjuvant effects of Virus-Like Particle (VLP) vaccine technology, spatial arrangement of bioactive components can significantly alter the behavior of these components in live systems. A new and promising way to implement nanoscale spatial organization is through DNA as a non-biological, self-assembling nanofabrication material. DNA nanofabrication, developed in the last 15-20 years, can easily arrange proteins, aptamers, and small molecules. Despite an explosion in popularity in academic communities, DNA nanofabrication has experienced limited industrial implementation. The unique capabilities, technical limitations, and notable challenges for DNA nanofabrication in an industry context will be discussed.