Low-Cost Method for Electrochemical Quantification of Hepatocellular Carcinoma Cell in Culture Media on Nanostructured Surfaces

J. Jaiswal, M. Dhayal
Indian Institute of Technology-Banaras Hindu University,
India

Keywords: cyclic voltametry, cellular interactions, biomolecules, low-cost

Summary:

In the present study, cyclic voltammetry (CV) assessment of human hepatic carcinoma cell suspension in culture media was performed to understand their interaction with biomolecules using three electrode system. Distinct cathodic and anodic peaks in the CV were observed for the culture media which attributes to the presence of a variety of biomolecules. The resulted response was optimized with different scan rates and the difference between the cathodic and anodic peak shift (Ep) obtained for estimating kinetics of electron transfer on the electrode surface. Slower scan rate shows the stability of charging and discharging characteristics which can be converted to slow kinetics of electron transfer on the electrode surface at higher scan rate. Further, the cell density was optimized with the linear decrease in both anodic and cathodic peaks currents. These cellular interactions were further validated by UV-Visible Spectroscopy and FTIR.