Hepatocellular Carcinoma Cells Response on Nanofunctional Films prepared through Time-Dependent Reduction Process in Physiological pH Environment

J. Jaiswal, M. Dhayal
Indian Institute of Technology-Banaras Hindu University,

Keywords: nanofunctional, glycan, reduction, hepatocellular carcinoma


Time-dependent UV-Vis spectroscopic analysis of gold nanoparticle (GNP) during the in-situ reduction process by natural glycan at their different concentrations was optimized in colloidal suspension. The reduction of gold chloride by natural glycan was successively achieved in both acidic (cationic environment) and basic pH (anionic environment). The FTIR spectra was used to quantify the relative change in nitrogen and oxygen atom containing functionalities indicating their role in the reduction process at different pH environments. 1H NMR spectra was further recorded to identify a relative change in corresponding peaks during the interaction of different functional moieties of chitosan with gold during the reduction process. We were able to achieve heterogeneous size distribution of GNP to monomial distribution within the natural glycan films with tunable surface properties at physiological pH. Further, it has been used to prepare functionally graded films by controlling gelification in time and temperature-dependent conditions. The presence of gold in the composite film was identified by X-ray diffraction peak with a gradual increase in peak intensity with an increase in natural glycan concentration at 2ϴ value of 38.1o and 44.3o which corresponds to the crystallographic planes (111) and (200) of GNP, respectively. A significant change in surface morphology has been observed in SEM images by addition of gold in polymer network. These nanofunctional films showed decreased viability and proliferation of human hepatocellular carcinoma cells (HepG2) at in-vitro conditions.