N.M. Bahadur, Y. Akter, R. Rashid, M. Islam, P. Haque, S. Alam
Noakhali Science and Technology University,
Keywords: wound healing, biodegradable polymer, composite film, mechanical properties, antimicrobial activity
Summary:This study was initiated to design a simple method for the preparation of a novel curative wound dressing film consists of natural polymers based on biodegradable gelatin and polylactic acid with silver nanoparticles where glutaraldehyde was used as compatibilizer. Silver nanoparticles prepared by chemical reduction were reinforced into the composite films and film formation was carried out using solvent casting technique. The shortcomings of pure components such as poor thermal and biological stability of gelatin and limited fluid retention capacity of PLA could easily be triumphed over by the prepared composite film. Silver nanoparticles were selected & incorporated into the film for preventing common clinical infections, and accelerating the rate of wound closure. The FT-IR technique confirmed the formation of composite through H-bonding and XRD showed the increase in crystallinity due to the incorporation of nano silver. It is noteworthy that the 50:50 (gelatin: polylactic acid) with 5% glutaraldehyde composite film showed the best elasticity & strength and excellent fluid retention property compared with pure components. SEM images showed the surface morphology of the films and presence of agglomerated Ag nanoparticles on film surfaces. Agar disk diffusion test against Staphylococcus aureus and Pseudomonas sp. in terms of zone of inhibition indicated appreciable antimicrobial efficacy of the composite films. Therefore, the new bioadhesive and biosensing wound dressing film had improved mechanical and thermal stability, sustainable antimicrobial effect along maximum cell viability. Finally, the in vivo mouse model showed maximum contraction within 12 days, which is more effective and faster than that of the conventional gauge bandages and able to respond with advanced sensing devices.