CCS Haryana Agricultural University,
Keywords: antimicrobial, activity, helianthus annuus nanomaterial, staphylococcus aureus
Summary:Abstract: Synthesis of some metals (Cu and Ag) nanoparticles has been carried out mediated by leaf extracts of some plants including castor oil (ricinus communis), neem (azadirachta indica), khat (catha edulis) and sun flower (helianthus annuus) as metal ions reductants as well as stabilizing agents. Aqueous metal salt solutions exposed to plant leaf extract led to the formation of metal nanoparticles in the size range 5-30 nm. The progress of bio-reduction of metal ions was monitored by observing their gradually increasing surface plasmon resonance peak intensity. As-synthesized nanomaterial was characterized by using XRD, TEM and FTIR techniques. The FTIR spectroscopic analysis suggested that metal nanoparticles, during their synthesis, are capped by the protein molecules present in the plant extract. Zones of inhibition for as-synthesized metals nanoparticles, measured at 24 hrs incubation, have shown their antimicrobial activity against some bacterial pathogens such as Gram negative Escherichia coli and Gram positive staphylococcus aureus.