Isonicotinylhydrazide on epigallocatechin gallate-protected gold nanoparticles: Toxicity management and stability enhancement

A. Umapathi, H.K. Daima
Amity University Rajasthan,
India

Keywords: Epigallocatechin gallate (EGCG), isonicotinylhydrazide, gold nanoparticles, stability, tuberculosis (TB)

Summary:

Tuberculosis (TB) is a menacing disease contributing to 1.3 million deaths annually1. It has triggered keen interest towards developing strategies to fight TB through distinctive approaches including tailored-made nanoparticles. The nanoparticles, due to their increased surface-to-volume ratio give rise to unique properties, and control over these physicochemical properties provide an opportunity to influence nano-bio interfacial interactions and subsequently their cellular internalization to manage TB infections2,3. In the current research, we have synthesized epigallocatechin gallate (EGCG) coated gold nanoparticles (AuNPsEGCG) and modified their surface with a first line anti-tuberculosis drug isonicotinylhydrazide (INH) to acquire AuNPsEGCG@INH. We anticipate that due to dual presence of EGCG and INH within a single system of AuNPs may trigger enhances therapeutic benefits towards TB, provided that both the biological agents are stable on the surface of nanoparticles. Therefore, we have evaluated the surface stability of EGCG and INH in bovine serum albumin (0.25%, analogous to the blood serum proteins) and in an-electrolyte NaCl solution (50mM), both in acidic and basic conditions. It was interesting to observe that the AuNPsEGCG and AuNPsEGCG@INH are stable in all the studied experimental conditions. Furthermore, this approach is useful to control the toxicity of free INH due to its stabilization on the surface and antioxidant (EGCG) moieties. References 1. Sulis, G.; Roggi, A.; Matteelli, A.; Raviglione, M. C., Tuberculosis: epidemiology and control. Mediterranean journal of hematology and infectious diseases 2014, 6 (1), 627. 2. A. Umapathi, Navya PN, H.K. Madhyastha, D. Jain, S. P. Srinivas, V. M. Rotello, H.K. Daima. Highly efficient and selective antimicrobial isonicotinylhydrazide-coated polyoxometalate-functionalized silver nanoparticles, Colloids and Surfaces B: Biointerfaces, 184, 2019, 3. Navya PN, H. Madhyastha, R. Madhyastha, Y. Nakajima, M. Maruyama, S.P. Srinivas, D. Jain, M.H. Amin, S.K. Bhargava, H.K. Daima. Single step formation of biocompatible bimetallic alloy nanoparticles of gold and silver using isonicotinylhydrazide. Materials Science and Engineering C: Materials for Biological Applications, 96, 2019, pp 286-294.