Lysozyme enriched tyrosine surface protected gold nanoparticles: Potent antimicrobials against multi-drug resistant bacteria

A. Umapathi, H.K. Daima
Amity University Rajasthan,
India

Keywords: Tyrosine, gold nanoparticles, lysozyme, MDR potency, toxicity

Summary:

The development of multi-drug resistance (MDR) in a range of bacteria towards available antibiotics is taking us back to the pre-antibiotic age. The occurrence of nosocomial infections is making the prosthesis implementations and the surgical interventions troublesome. Therefore, there is an urgent need to counter these problems, and designer made functional nanomaterials are getting attention due to their improved pharmaceutical potency and unique physicochemical properties (1,2). In the present research, we demonstrate a strategy to prepare tyrosine amino acid protected gold nanoparticles (AuNPsTyr) (3,4), which are further enriched with lysozyme to develop higher potency towards multi-drug resistant bacteria. These nanoparticles are characterised for their size, shape, surface charge, hydrodynamic radius, and optical properties. Stability of these nanoparticles are confirmed in a range of biological fluids, and bacterial growth media. Currently, we are investigating the MDR potency of these nanoparticles against a range of microbial systems. Additionally, the toxicity of these (AuNPsTyr and AuNPsTyr@Lyso) nanoparticles are being assessed on mouse fibroblast cells. References: 1. Navya PN, H.K. Daima. Rational engineering of physicochemical properties of nanomaterials for biomedical applications with nanotoxicological perspectives. Nano Convergence, 3 (1), 2016, pp. 1-14. 2. H.K. Daima, V. Bansal. Chapter-10: Influence of physico-chemical properties of nanomaterials on their antibacterial applications, Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases, 2015, pp. 151-166. Book published at Boston by Academic Press/Elsevier, edited by Mahendra Rai and Kateryna Kon. 3. K. Dubey, B. G. Anand, R. Badhwar, G. Bagler, Navya PN, H.K. Daima, K. Kar. Tyrosine- and tryptophan-coated gold nanoparticles inhibit amyloid aggregation of insulin. Amino Acids, 47 (12), 2015, pp. 2551-2560. 4. H.K. Daima, PR. Selvakannan, R. Shukla, S.K. Bhargava, V. Bansal. Fine-tuning the antimicrobial profile of biocompatible gold nanoparticles by sequential surface functionalization using polyoxometalates and lysine. PLoSOne 8 (10), 2013, pp. 1-14.