Y. Li, W. Cui, H. Jin Yoo, C. Baek, J. Min
Keywords: pathogen concentration, large volume sample, lab-on-a-chip, graphene oxide
Summary:An effective method for detecting waterborne pathogens in large volume of water is seriously needed for hygiene control. However, traditional methods, such as culture & counting, generally take long detection times and low sensitivities. Therefore, various methods such as molecular diagnostics and immunoassay have developed to overcome these huddles. These methods have good sensitivity and selectivity with target amplification or signal amplification. However, current molecular diagnostic methods, which use only small amounts of samples, require concentration of the samples to increase analytical sensitivity. Here, we developed a microfluidic device for waterborne bacteria (S.aureus and S.typhimurium) concentration in large volume of water. This chip includes sample concentrating chamber with micro beads coated by graphene oxide which has a huge number of functional groups can adsorb bacteria in aqueous states. The developed microfluidic chip successfully concentrates pathogens in 10 mL solution and it can be applied to enhance sensitivity of various analytical biosensors for large volume of aqueous sample.