Quercetin synthesized amylase capped metal nanozymes for industrial applications

P.N. Navya, S. Sanjana, M.U. Medha, R. Madhyastha, H. Madhyastha, S.P. Srinivas, M. Maruyama, H.K. Daima
Siddaganga Institute of Technology,

Keywords: quercetin, metal nanozymes, gold, silver, nanoparticles, industrial


Preparation of nanomaterials for industrial, environmental, medical or biological applications necessitate a profound understanding of nano-bio interface. Interaction of metal nanoparticles with biological moieties and industrial products critically depends on their physical and chemical properties. Consequently, the dynamic surface properties and composition of metal based nanoparticles must be sensibly designed for explicit applications (1-4). In present research, we have formulated gold (Au), silver (Ag) and their bimetallic Au-Ag nanoparticles with different ratios using ‘quercetin’ to control their metal composition, and further surface modified with enzymes as shown in Figure 1. The key reasons why we have used quercetin as reducing and stabilizing agent are (a) quercetin has reducing groups that can reduce metal ions into their atoms; (b) their bio-importance; and (c) structured shell of quercetin bound to the surface of nanoparticles helps in anchoring these nanoparticles with other biomolecules such as enzymes. Moreover, employing quercetin for nanoparticles synthesis is a major step towards eco-friendly assembly of bimetallic nanoparticles of different compositions in single step. The nanoparticles fabricated during this study have been characterized by UV-Vis spectrometry, Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM) and Zeta potential analyzer. The enzyme characterization studies were carried out to check for the optimal pH and temperature. Furthermore, the reusability of the functionalized enzyme and its application was studied for monometallic Au and Ag nanoparticles. The results indicated that quercetin reduced Au and Ag nanoparticles can be effectively used as a matrix to functionalize enzymes of industrial importance.