Biologic targeting of nanozymes through biomolecular surface corona

H.K. Daima
Amity University Rajasthan,
India

Keywords: gold, silver nanoparticles, surface corona, biomolecules, in-vitro nanozyme, biological

Summary:

Gold and silver based formulations are known since antiquity for medicinal, ornamental and cosmetic applications. Though, deeper understanding of these metals at nano-level has attracted more attention in recent past due to their unique physicochemical and biological targeting properties. Metal nanoparticles are well-known to exhibit size, composition, shape and surface charge-reliant biomedical profile (1-5). Nevertheless, to this end, usage of nanoparticles by altering their surface corona with biomolecules and investigation on their in-vitro biological behavior has not yet been examined extensively. Therefore, in current work, we have used green and eco-friendly method to prepare biomolecular surface corona on gold and silver nanoparticles to compare their inherent in-vitro biological behavior. In a single step, we have equipped gold (Au) and silver (Ag) nanoparticles with tyrosine (Tyr), tryptophan (Trp), isoniazid antibiotic (Iso), epigallocatechin gallate (EGCG) and curcumin (Cur), respectively. Optical properties, presence of various biomolecules on the surface of gold and silver nanoparticles, their negative surface charge, metal content and hydrodynamic radius have been confirmed by UV-visible absorption spectroscopy, FTIR, zeta potential, AAS and DLS analysis, respectively. Furthermore, the governing role and targeting potential of these nanoparticles have been evaluated with respect to biomolecular corona by free radical scavenging capacity, in-vitro nanozyme activity and antimicrobial action.