Organic Nanobiomimetic Memristor/Memcapacitor Devices Ultrasensitive Direct Detect Matrix Matelloproteinase-2 in Human Serum

E.T. Chen, J. Thornton, S-H. Duh and P.T. Kissinger
Advanced Biomimetic Sensors,
United States

Keywords: organic memristor/memcapacitor, nanobiomimetic crossing-nanotubes, inducing bio-communication, direct electron-relay transfer system, energy storage and sensing device, MMP2, probe-free and label-free


Matrix Matelloproteinase-2 (MMP-2) plays a key role in many diseases. A new type of dual-functioning device was developed for fast, direct ultrasensitive detection of MMP-2 using NIST SRM965A reference human serum specimens under label-free, probe-free and reagent-free conditions. The organic memcapacitor/memristor device was based on a self-assembled polymer membrane having polarized 3D array crossing-nanotube structures on a gold substrate that enables selective inducing bio-communication with MMP2. The characteristics of memcapacitor/memristor were observed in the i-V curves of the hysteresis. The Chronoamperometry (CA) method has a Detection of Limits (DOL) value of 8.67x10-18g/mL in PBS solution related to current density between 1.47 µA/cm2 and 919.2 mA/cm2 over MMP2 concentration between 20 ag/mL to 100 ng/mL with a Relative Pooled Standard Deviation 1.4% (n=26). The Double Step Chronopotentiometry (DSCPO) method produced a similar impression value of 1.47% (n=18) over MMP2 concentration 40 ag/mL to 100 ng/mL over energy density between 185 - 0.47 µWHR/cm3. The sensor was able to direct detect MMP2 in pure NIST serum specimens in the concentrations of 81.15 ± 0.10 ag/mL in normal glycemia serum, 1.13 ± 0.0016 pg/mL in hypoglycemia serum and 1.4± 0.0001 pg/mL in hyperglycemia serum, respectively. Multiple factors impacting on the sensor’s energy storage capability were also studied. (ACKNOWLEDGEMENT) The authors would like to thank NFC and Lin’s MBE lab (Nanostructure and Quantum Device Laboratory) in NCTU, Taiwan for sample supported.