A Noninvasive Fluorescent Biosensor for Transdermal Glucose

L. Tolosa, S. Brown, C. Tiangco, E. Corson
University of Maryland Baltimore County,
United States

Keywords: noninvasive glucose sensing


Our group has been developing a painless, noninvasive method of collecting glucose spontaneously/passively diffusing through the skin. The transdermal glucose collected in this manner has a concentration in the μM range, thus we developed a reliable glucose sensor that can measure glucose at these levels. Here we describe a fiber optic biosensor for μM glucose that is based on the glucose binding protein (GBP) labeled with either BADAN in the H152C position or acrylodan in the L255C position and immobilized on Ni-NTA agarose beads via metal-histidine interaction. The portable, low-cost biosensor system consists of an optical fiber with the sensitized beads trapped on one end, and appropriate optics and electronics on the other end. The control software and the visual interface for the optical sensor is designed and implemented in LabVIEW and runs on a tablet computer. To collect the transdermal glucose, an automated system with a sampling head attaches to the skin surface and executes a wash, dry and sampling protocol. Data collected from several human subjects using our noninvasive glucose monitoring system will be presented.