Impact of altered biomolecular surface corona of gold and silver nanoparticles on their in-vitro biological behavior

P.N. Navya, M.R. Meghana, T.S. Shruthi, A. Umapathi, D. Jain, H. Madhyastha, H.K. Daima
SIT, Tumkur,

Keywords: metal nanoparticles, surface corona, biomolecules, in-vitro, biological


In various part of the world the medicinal, ornamental and cosmetic potential of gold and silver based formulations are known since ancient times. However, the recently developed deeper understanding on these metals at nanoscale level has attracted more attention due to their unique biomedical influence with more specificity and efficacy. In general, metal nanoparticles are well-known to exhibit size, composition, shape and surface charge-reliant biomedical profile (1-5). However, to this end, use of inorganic gold and silver nanoparticles by altering their surface corona with biomolecules and investigation on their in-vitro biological behavior has not yet been examined extensively. Therefore, in this work, we have used green and eco-friendly routes over conventional chemical methods to prepare biomolecular surface corona on gold and silver nanoparticles to compare their inherent in-vitro biological behavior. We have synthesized gold and silver nanoparticles by using tyrosine (Tyr), tryptophan (Trp), isoniazid antibiotic (Iso), epigallocatechin gallate (EGCG) and curcumin (Cur), respectively. Optical properties, presence of various biomolecules on the surface of gold and silver nanoparticles, their negative surface charge, metal content and hydrodynamic radius of various nanoparticles have been confirmed by UV-visible absorption spectroscopy, FTIR, zeta potential, AAS and DLS analysis, respectively. To confirm the impact of biomolecular corona of nanoparticles; free radical scavenging capacity, in-vitro enzyme-like activity and inherent antimicrobial nature of these biomolecular surface corona containing metal nanoparticles are currently being evaluated.