nano-oxygen carrier for machine perfusion organ storage

G. Srinivas
TDA Research Inc.,
United States

Keywords: machine perfusion, organ storage, perfusate, oxygen carrier


TDA Research, Inc. (TDA) and its collaborator Southwest Research Institute (SwRI) are developing a perfusion system for the salvage and repair of trauma injured tissue. Machine perfusion is an emerging preservation modality for tissue preservation that uses an ex vivo system to deliver an oxygenated perfusate to a tissue sample or organ to preserve metabolic activity, potentially repair tissue, and increase storage time. The shelf life of implantable tissues stored via static cold storage is generally in the range of 4-8 hours, machine perfusion tissue storage has demonstrated a shelf life of over 100 hours. The further development of successful perfusion systems will greatly benefit the growing need to preserve and repair tissue and organs, with the goal of eventual banking capabilities for tissue and organs. The key to our novel perfusion technology will be the combination of a ruggedized, portable machine perfusion system, and a novel perfusate being developed by SwRI. SwRI’s perfusate contains a novel, universal oxygen carrier system that avoids the need to use actual blood as the perfusate, which is in short supply and also presents problems of blood-type matching and subsequent immune-rejection of the transplanted tissue. The use of a perfusate with a universal oxygen carrier is essential to a successful normo- or sub-normothermic machine perfusion system for preservation of tissue. Furthermore, the perfusate studied in this work mimics blood, providing nutrition and oxygen to maintain the normal metabolism of transplantable tissue. In this project, TDA and SwRI are developing an improved, novel perfusate for the preservation of tissue. SwRI is working to optimize their perfusate via nano-modifications to the surface to maximize circulation time while minimizing toxicity. Concurrently, TDA is working to design the ruggedized, portable machine perfusion device that will be able to use this improved perfusate in the field. TDA's ruggedized perfusion system design will incorporate a suite of sensors including temperature, pressure, and O2 concentration. These sensors will allow the perfusion system to operate with automatic control and will alert users if the tissue has been exposed to unacceptable conditions. At the end of the Department of Defense funded project, TDA and SwRI will have demonstrated a working breadboard prototype model that will be capable of circulating the perfusate in a vascular tissue sample. Details of the experiments and results will be presented.