Evaluation of the citotoxicity of TiO2 modified on Cervical Cancer line-cells- Hela

M.J. Basante, O. Gutierrez and R.J. Camargo
Universidad Del Valle,

Keywords: innovative, oncology, treatment


Titanium dioxide nanoparticles are increasingly being used in pharmaceuticals, cancer therapies and cosmetics due to the unique photacatalytic properties and their small sizes. The modified TiO2 a substance with potential and future uses in the treatment of various cancers. The alternative photodynamic therapy with TiO2-Au, TiO2-mod-FA, TiO2-mod nanocomposites was studied on Cervical Cancer line-cells HeLa and Non-cancerous cells - CHO line cells. Nanocompounds of TiO2-mod were synthesized using a sol-gel route. The characteristic functional groups of each material were analized by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR), the sintetizing process no showed contamination this result was obtained by Energy Dispersive Spetroscopy (EDS). The structure of the each nanomaterial was studied by X-ray diffraction (XRD). The concentrations of the nanocomposite, the UV-A exposure times and UV-A presence or absence were manipulated like variables. The Cytotoxicity Detection Kit (LDH) showed 99% of cytotoxicity in HeLa cells but it did not reveal cytotoxicity in CHO cells. Furthermore, the time and concentration for the treatment were significantly reduced compared to other traditional therapies. For instance, it was only needed 40 minutes and a concentration of 200 ppm; showing that this alternative therapy is much more effective. Conclusions: The selectivity for cancer cells lines was the principal finding, this finding open a further possibility for the use of these nanomaterials in cancer.