S-H. Duh, J. Thornton, P.T. Kissinger, E.T. Chen
advanced biomimetic sensnors, inc,
Keywords: nanobiomimetic memristor/memcapacitor, reversible membrane potential, slow-wave-sleeping, voltage sensor, reagent-free, LPS
Summary:Lipopolysaccharide (LPS) is a common endotoxin from E. Coli bacteria, and is the major source causing infection diseases over 20 million people worldwide. Lack of sensitivity and high imprecision from common commercial test methods are associated with the protein interference and plus time consuming assay and sample preparations led to a paramount challenge. We report developed a nanostructured biomimetic sensor for direct measure sub pg LPS in human and organic milk designated for infants, under the conditions of free from antibody and radio labeling tracers, and is totally reagent-free. The gold sensor ships have nano island structure membrane with cross-linked polymers and has function groups mimicking Fibroblast Growth Factor Receptor 1 (FGFR1), it can measure the signal changes of LPS by a double step chronopotentiometry (DSCPO) method over a linear concentration range up to 0.5µg/mL in 40 µL specimen samples with a Detection of Limits (DOL) of 0.3 ng/mL over the energy density range between 123.2 and 0.11 µWHr/cm3 using human milk specimens at 0.25 Hz and ±10 nA with an imprecision value 3.0% (n=12) against 9.8 to -0.042 µWHr/cm3 for organic milk samples. Organic milk samples do not have a LPS concentration correlated relationship to the energy density change, indicating it is not responsive when LPS attacking the host at SWS. The impact of different type of milks upon the integrity of the reversible membrane action/resting potential was accessed during slow-wave-sleeping (SWS) 0.25 to fast gamma 200 Hz. The results indicate human milk having orders of magnitude sensitive to detect energy change than organic milk at SWS for with or without LPS at 0.25Hz. The results demonstrated human milk in promotion of brain development and memory than organic milk for infants. This work was built upon our previous works: Nanostructured Memristor Sensor Mimics Acetylcholinesterase (ACHE) Active Sites In The Gorge For fM Detection Of Acetylcholine, NSTi-Nanotech, 2, 200-203, 2014. A Nanobiomimetic Neuronal Memcapacitor Serves as a Voltage Sensor and an Amperometry Sensor for Reagent-less Direct Detection of Sub pM Soluble Amyloid-beta. Biotech, Biomaterials and Biomedical: TechConnect Briefs, 172-175, 2015. Nanobiomimetic Memcapacitor Memory Devices Identify Circadian Rhythm Dysfunction and Predict Early Signs of “Epilepsy” Using Reentrant Energy-Sensory Images, Advanced Manufacturing, Electronics and Microsystems: TechConnect Briefs, 200-203, 2015. Acetylcholine Repairs the Amyloid-beta Damage on Brain Circuitry and Memory Loss From a “Mutated Biomimetic Acetylcholinesterase” Neuronal Memcapacitor During Slow-Wave Sleeping, Advanced Manufacturing, Electronics and Microsystems: TechConnect Briefs, 226-229, 2015. The new accomplishments are:1) Orders of magnitudes increased the detection sensitivity compared with the data reported in literature for LPS;2) The precision also improved a magnitude using milk specimen compared with 30-50% error reported in literature; 3). Our assay is superior compared with the conventional assay in shortened test time by 104-fold, Reagent-free without environment contamination, easy and burden-free benefit for workers; simple and portable;4). It will open wide market applications.