F. Yang, M.H. Rafailovich, T. Mironava
Stony Brook University,
Keywords: gold nanoparticles, dermal fibroblasts, nanotoxicology
Summary:As human age there are many significant changes that occur in the body. Changes in the skin are the most prominent and include not only alteration of biochemical composition but also modification of cellular mechanics. The last one is of the particular interest considering its importance for correct physiological functioning. Here we investigate how the age-dependent changes in dermal fibroblast mechanics affect cell response to the nanoparticles. To address this issue, the AFM technique was used to examine the rigidity of fibroblasts isolated from 6 human donors of different age groups. To analyze how the mechanical behavior of dermal fibroblasts affects cell response to the nanoparticles we exposed cells to gold nanoparticles (AuNPs) of two different sizes (17 nm and 36 nm) and performed mass spectroscopy analysis, confocal and proliferation studies. AuNPs have an enormous potential in chemical sensing, biological imaging, drug delivery, and cancer treatment. Therefore, it is important to know their potential toxicity to cells as a function of age-dependent cell mechanics. We found that there are significant differences in the membrane rigidity, overall nanoparticles uptake, and NPs toxicity among neonatal, 80-years old and 30-years old cell groups confirming that the effect of NPs exposure depends not only on concentration and time but also on patient age.