Y. Xu, M. Rafailovich, T. Mironava
Stony Brook University,
Keywords: nanotoxicology, titanium dioxide
Summary:The importance of titanium dioxide nanoparticles (TiO2 NPs) stems from its ubiquitous applications in personal care products, solar cells and food whitening agents. Hence, this NPs come in direct contact with the skin, digestive tracts and are absorbed into human tissues. Currently, TiO2 is considered as safe commercial ingredient by the material safety data sheets with no reported evidence of carcinogenicity or ecotoxicity, and do not classify as a toxic substance. This study examined the direct effects of TiO2 on human Adepose Derived Stromal Cells (ADSCs) one of the cellular components of adipose tissue. Specifically, we studied the interaction of ADSCs with two forms of TiO2 NPs - rutile and anatase. We focused on the effects of TiO2 exposure on intracellular lipid accumulation and expression of differentiation markers; on whether different forms of TiO2 have similar effects on cell function; and whether nanoparticles localization inside cells correlates with loss of cell function. In addition presence of bacteria on the skin is taken into account in a complex interaction among ADSCs, TiO2 nanoparticles, and bacteria. Altogether, our findings indicate that nanosized TiO2 adversely effects differentiation of ADSCs, have profound effects on cell function and increase the rate of bacterial infection.