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Beyond Biomarkers: Array-Based Profiling for Diagnostics and High-Throughput Screening
V. Rotello
University of Massachusetts, Amherst, US
Keywords: medical diagnostics, sensor array
Summary:
The "chemical nose/tongue" approach presents a potential alternative to specific recognition and separations techniques. In this approach a sensor array is generated to provide differential interaction with analytes via selective receptors, generating a stimulus response pattern that can be statistically analyzed and used for the identification of individual target analytes and also analysis of complex mixtures. Recently, we have developed nanoparticle-GFP based “chemical nose” strategy for protein detection in biofluids. We have used this system for sensing in serum, and used cell lysates to detect and identify cancer in vivo. We have extended our array-based approach to cell surface sensing, demonstrating rapid differentiation of isogenic healthy, cancerous, and metastatic cell lines. Recently, we have extended this method to high content screening of diagnostics, allowing the rapid determination of both efficacy and mechanism of drug action in a "one-well, one drug" platform that can greatly expedite drug discovery and toxicology research. .
