Conductive Polymer Microelectrodes for on-chip measurement of transmitter release from living cells

S. Tylsgaard Larsen, M. Matteucci, R. Taboryski
Technical University of Denmark, DK

Keywords: electrochemical detection, transmitter, conductive polymer, PEDOT, electrode, microfluidics, exocytosis


In this work we present an all-polymer chip for measuring exocytotic release from neuronal cells using electrochemical sensing at a conductive polymer microelectrode. This chip combines several novel aspects, such as the use of conductive polymers in biosensing, a pressure-based cell capturing technique, and a method of measuring comparable transmitter release signals from large groups of cells. Amperometric signals from passage 12 rat pheochromocytoma cells were measured to demonstrate the function of the chip. The presented device is cheap, easy to fabricate, and can be used for pharmacological screening applications. Furthermore Pedot:tosylate is a very promising electrode material in chip-based devices for several bioanalytical applications such as HPLC, capillary electrophoresis, and drug screening.