Water-soluble nanoparticles as an universal imaging tool

J. Niehaus, S. Becker, C. Schmidtke, D. Ness, K. Werner, H. Weller
Center for Applied Nanotechnology, DE

Keywords: water soluble nanoparticle, functionalization, phase transfer


Here we present a two step concept for phase transfer of NPs soluble in non-polar media. The first step is an exchange of the stabilizing organic molecules against a multidental binding short chain polymer. In a second step the modified NPs are encapsulated in block-copolymer micelles and transferred into an aqueous solution. Finally we close the micelle shell by cross linking the polymer chains to ensure a good separation of NPs and surrounding media. The encapsulation is performed by using micro fluidic components in a continuous flow phase transfer approach. The opportunity of using block-copolymers with different end-groups gives us the possibility to functionalize our micelles with affinity molecules like antibodies. We will show in vitro and in vivo measurements of functionalized and unfunctionalized particles where the affinity to the selected targets can clearly be seen. In addition this encapsulation procedure can be used for different kinds of nanoparticles without changing the interaction between the outer shell of the capsule and the surrounding. This makes our system ideal for cross evaluation of novel methods like MPI.