Ultrastructure of hepatic cells in rabbits after injection of nanoparticles MCS-B

A.N. Belousov
Laboratory Applied Nanotechnology, UA

Keywords: ultrastructure, hepatic cells, nanoparticles, MCS-B


In order to determine ultrastructural reconstructions in hepatic cells under effect of nanoparticles of magnetite (MCS-B), an experiment was conducted on 45 rabbits. Ultrastructural changes in hepatocyte organelles manifested pronounced signs of activation of reparative intracellular processes. Single ribosomes were found on the outer membrane of the nucleus.Mitochondria were evenly distributed in all parts of the cytoplasm of the hepatic cells. Many cristae were revealed; they had a pronounced typical orientation. The outer membrane remained integral, without any foci of destruction. The laminated cytoplasmic Golgi’s complex was moderately hypertrophic, its membrane part consisted of parallel smooth membranes. Packs of these membranes were surrounded with a great number of large and small vesicles. There was rather a great number of primary lysosomes in the area of localization of the laminar cytoplasmic Golgi’s complex, autophagosomes and small inclusions of lipids being observed in single cells. Sinus capillaries and Disse’s spaces were dilated rather extensively. Disse’s spaces were filled with numerous microvilli. Karyolemma had no destructive changes. The cytoplasmic membrane did not undergo any changes and held its well-defined bilaminated structure. It should be noted that there was a great number of small electron-transparent micropinocytic vesicles.