Copper complexes loaded on nanostructured TiO2 materials as citotoxic agents of cancer cells

T. Lopez, E. Ortiz, P. Guevara, E. Gómez
Instituto Nacional de Neurologia y Neurocirugia, MX

Keywords: copper complex, nanostructured TiO2, cancer agent


In the present work ammonium tetrachlorocuprate (II) and copper (II) chloride were loaded on nanostructured mesoporous titania for determinate their anticancer properties. Copper-TiO2 materials were prepared using the sol-gel technology. Characterization of Copper complexes and Cu-TiO2 materials was carried out by different physical-chemical techniques. The cytotoxic properties of both pure complexes as these loaded on titania were determined using the rat glioblastoma RG2 cell line. The FTIR and UV-VIS spectroscopy indicate that copper complexes were success loaded on titania without changes in their structures. The surface area obtained from the N2 adsorption-desorption isotherms were of 515, 205 and 226 m2/g for TiO2, Cu(NH4)2Cl2-TiO2 and Cu(Cl)2-TiO2 respectively. Release profiles shown two steps of copper complexes released. RG2 cells were exposed to copper complexes and Cu-TiO2 materials at different concentrations and 24 hours post treatment the cell viability was tested by MTT assay, which measures mitochondrial activity. This technique provides qualitatively the cell survival only seeing if there is a color change. We observed that when the cells were treated with copper complex alone and copper complexe-TiO2 there are stronger coloration yellowing indicative that these compounds killed the cell and therefore MTT couldn’t be reduced by the mitochondria.