Comparison of Anchoring Materials for High Performance Sensors Modified with Nanoparticles and Enzymes

X. Gao, D. Assan, J.C.K. Lai, S.W. Leung
Idaho State University, US

Keywords: performance, anchoring material, nanoparticle, sensor, bioenzymes


We have previously developed some of the most sensitive biosensors/electrodes modified by Au nanoparticles and enzymes that are capable of detecting concentration levels below ppb. These sensors were fabricated with composite layers of nanomaterials and enzymes anchored on conductive but non-reactive materials, such as glassy carbon Au, Ag, and Pt. Performance of these sensors varies depending on the anchoring materials and as well as the composition of the biocomposite materials. In this report, we investigated the performance of sensors fabricated with identical biocomposite materials and procedures, except the anchoring conductive materials. The anchoring materials were glassy carbon, Pt, Au and Ag; the biocomposite layer consisted of polymer/Au nanoparticles/enzyme. The enzymes in the biocomposite layers are essential for the target species detection with which enable the coupling (detection) reactions occur. The enzymes used in this study were LDH, GDH, and hemoglobin. The specific target species for the detection included lactate, NO2-, peroxide, and NH4+. We also tested different combinations of the biocomposite layers for optimal performance of the sensors/electrodes. The testing solutions were buffered with 0.1 M of phosphate at pH 7.0.