Lyophilisomes: novel protein capsules as drug delivery system

E. van Bracht, P.J. Geutjes, R.G. Wismans, E. Oosterwijk, T.H. Kuppevelt, W.F. Daamen
Radboud University Nijmegen Medical Centre, NL

Keywords: lyophilisomes, drug delivery, Doxorubicin


Drug delivery capsules are carrier materials designed for improving the biodistribution of systemically applied (chemo)-therapeutics. This study describes a novel nano/microcapsule (named ‘lyophilisomes’) made by a three step procedure comprising freezing, annealing, and lyophilisation. Here, albumin lyophilisomes were prepared with a size ranging from 100 up to 3000 nm. When exposed to HeLa cells, they did not affect the cell viability and confocal microscopy showed internalization, indicating its carrier potential. To probe the possibility to use lyophilisomes for tumor targeting, they were loaded with doxorubicin. Release studies showed a minimal release (less then 2,1%) and when SKOV-3 cells were exposed to doxorubicin loaded lyophilisomes a dramatic decrease in cell viability to 6±2% after 72 h was observed, emphasizing the drug delivery potential of lyophilisomes.