Anticancer activity of oligosaccharides extracted from Capsosiphon fulvescense using enzyme coated nanofibers

H-W Kim, J-H Lee
Chosun University, KR

Keywords: nanofiber, enzyme, oligosaccharides


Dried C. fulvescens was ground and sieve through a 200 mesh. A 100 mg of C. fulvescens was homogenized with 100mL of Buffer, and then 10 mg of alcalase coated nanofiber was mixed. The enzymatic hydrolysis were performed for 12h at 50℃. The enzyme hydrolysates prepared was used to test anticancer activity. The alcalase hydrolysate was separated by UF membrane system(MWCO 2000, Hydro-2K, Sartorius). The total carbohydrate of the below MWCO 2000 fraction was determined by the phenol-sulfuric acid method at 490 nm. Alcalase hydrolysate contained 67.1% total carbohydrate, 29.8% uronic acid, 0% sulfate, and 3.1% protein. A below MWCO 2000 contained 2.8% protein, 42.9% uronic acid, 0% sulfate, and 54.3% total carbohydrate, which is mainly composed of glucose(95%). Anticancer cells line (HeLa cell, HCT116 cell, and MKN45 cell) were maintained in RPMI 1640 medium containing 10% heat inactivated fetal bovine serum supplemented with penicillin (100 U/ml) and streptomycin (100 μg/ml) at 37℃ under 5% CO2 in the air. The viability levels of Anti-cancer activity were determined by MTT assay. HeLa cell, HCT116 cell, and MKN45 cell exhibited the highest inhibitory effect at 1000ug/ml where the Alcalase hydrolysate was 41%, 38%, and 49%, respectively.