Novel Immune Biosensors Based on the Structured Nano-Porous Silicon for Control of Mycotoxins in Environmental Objects

N.F. Starodub, N.F. Slishek, I.V. Pylypenko, L.N. Pylypenko
National University of Life and Environmental Sciences of Ukraine, UA

Keywords: silicon, nanostructured, immune biosensor, mycotoxins, determination


To meet all the requirements of practice in respect of high sensitivity of analysis of mycotoxins as well as simplicity, cheapness and rapidity of its currying out we propose to use of structured nano-porous silicon (sNPS) as transducers for the immune biosensors with the registration of the specific signal on the basis of changes of chemiluminescence or photocurrent of this structure. The information concerned investigations of some physical-chemical abilities of sNPS, worked out algorithm of analysis, obtained results and possible mechanism of specific signal formation is main goal of this report. We used boron doped single-crystal silicon with resistivity of 1 Ohm/cm, area of 100 cm2 and thickness of 0.3 m. sNPS layers were prepared with help of HF:HNO3 solution. T-2 mycotoxin and patulin were used as model of low molecular weight toxins. The analysis was curried out by “direct” way when specific antibodies (Ab) were immobilized on the sNPS surface and then they reacted with appropriate mycotoxin. The sensitivity of such biosensors is on the level 10 ng/ml. It is open perspective for the practical application of the proposed immune biosensor for the determination others types of toxic substances with the use of their specific Ab.