The Effects of Temperature and Relative Humidity on the Stability of Paper-immobilized Antibodies

J. Wang, B. Yiu, J. Obermeyer, C.D.M. Filipe, J.D. Brennan, R.H. Pelton
McMaster University, CA

Keywords: bioactive paper, antibody stability, antibody printing, immobilized antibodies, ELISA


In the past 5 years there has been a significant research effort focused to the development of inexpensive paper-based sensors that can detect a range of analytes rapidly in resource-limited settings. One of the challenges related to incorporating biosensors into packaging and other consumer products is that the biorecognition agent must survive the manufacturing process and have a useful shelf life. At present there are no literature reports on protein deactivation during high speed printing, and in particular on the effects of high temperature drying associated with roll-to-roll printing operations. There is also little data available on the best conditions for storage of bioactive paper products. Therefore, we report results of a detailed study on the influence of temperature and relative humidity on the deactivation rate of antibodies immobilized on paper, with the goal of understanding how printing processes and different storage conditions would affect antibody stability. Our results were also fit to an empirical equation and we demonstrate the use of this model to predict the extent of antibody deactivation when the antibody is printed and dried with a commercial printing operation, and when the antibody is stored at different temperatures and humidity levels.