Self-Separation of Blood Plasma/Serum During Capillary Flow of Whole Blood in the Microchannel

B. Babu Nunna, L. Narayanan Venkatesan, S. Zhuang, H. Singh, E.S. Lee
New Jersey Institute of Technology,
United States

Keywords: self-separation, capillary flow, blood plasma, erythrocytes, plasma separation, microchannel


Most of the clinical chemistry tests and diagnostics are performed on the cell-free serum or plasma of the whole blood. Therefore the blood tests performed on the micro-devices needs an integrated on-chip plasma/serum separation from the whole blood. The separation of the blood plasma/serum during the capillary flow in microchannel is achieved by optimizing the hydrophilicity of the microchannel which controls the flow velocity of blood in microchannel. The change in the shear effect on RBCs caused by the change of the velocity field of serum due to capillary force causes the difference in the velocities of the particles (RBCs) and serum, which leads to the difference in the moving distance or serum separation. In this research, the relation between the hydrophilicity of microchannel and separation phenomenon was established by measuring the separation volume (Serum/ plasma volume flowing ahead of the RBC in the microchannel) was measured for microchannels with different hydrophilicity for blood samples with constant hematocrit (~40%).