An Innovative Multiplex Assay with Point-of-Care (POC) Nano Biochip for Enhanced Cancer Screening

B. Babu Nunna, E.S. Lee
New Jersey Institute of Technology,
United States

Keywords: cancer Screening, tumor biomarker detection, nano sensing, gold nano particles


The inexpensive nano biochip takes advantages of its unique sensing technology in exclusive microfluidic platform, which leads to detect the multiple tumor biomarkers in the patient bodily fluids and produce instantaneous results. The novel biochip with multiplex assay in the microfluidic platform detects multiple tumor biomarkers with dynamic range of concentration in the biofluid sample and generates instant results at the physician’s office, which the current available detection methodologies cannot perform due to the limitations like sample preparation, sample volume, limit of detection and duration of analysis. The continuous monitoring of the cancer biomarkers levels helps oncologists to understand the cancer progression and to make the decision for the next level of examination and further required treatment in order to manage cancer more efficiently. The tumor markers tested on the biochip are cancer antigen CA-125 and Human Epididymis protein HE-4. Though cancer antigen CA-125 (in μg/ml concentration) shows elevated levels for ovarian cancer (OVC) patients, it is also expressed in some non-malignant cases. But Human Epididymis protein HE-4 (in ng/ml concentration) is mostly restricted to malignant OVC cases with the sensitivity and specificity of 67% and 96% respectively. HE-4 in conjunction with CA-125 has enhanced sensitivity to 76% and specificity to sustain at ~96%. The cancer antigens when flow in the microfluidic platform, conjugate with the antibodies that are immobilized on the sensing platform using carboxylic gold nano particles. The changes in the dielectric constant of the media due to the antigen-antibody conjugation change the capacitance in the interdigitated electrodes of the sensing platform in the biochip. The details of the change in the electrical properties like capacitance, due to the biological interactions in the microfluidic flow are studied for detecting the multiple tumor biomarkers.