Developing an approach to identify silver-responsive gene: in silico analysis by Connection up- and down-regulation expression analysis of microarrays (CU-DREAM)

A.W.K. Sooklert, D. Jindatip, N.W.S. Cherdchom, A. Sereemaspun
Chulalongkorn University,
Thailand

Keywords: silver nanoparticles, cytotoxicity, gene expression, in silico

Summary:

Currently, silver nanoparticles (AgNPs) are one of the most nanoparticles used in innovative development. AgNPs have been widely used, and people exposed to the particles via several paths mainly through direct contact, inhalation, and ingestion. Previous studies have shown toxic effects of AgNPs exposure in many mammalian cell types. However, nanosilver-responsive genes have not been clearly elucidated. By acknowledging these unwanted outcomes, the early detection and prevention of nanotoxicology at gene level could be implemented. In this study, we identified the changes in gene expression level that could be potential indicators for AgNPs toxicity. Here, we used in silico method, Connection up- and down-regulation expression analysis of microarrays, to detect possible nanosilver-responsive genes in xenogenic targeted organs. From Gene Expression Omnibus (GEO) Series database, we selected genes which were up-regulated in AgNPs, but down-regulated in silver ion as nanosilver-responsive genes. In conclusion, our results represented gene data after AgNPs exposure. This research is building to provide insight how we can access and predict the toxicity from nanoparticles exposure.