Rapid Bacteria Isolation and Collection for Infection Diagnostics without Culture

Y. Xu, J. Struss, T. Ferguson, D.T. Hung, Z. Zhou
Luna Innovations Inc.,
United States

Keywords: magnetic nanoparticles, bacteria, blood, culture

Summary:

Rapid Bacteria Isolation and Collection for Infection Diagnostics without Culture Yang Xu a, Jeffrey Struss a, Tammy Ferguson a, Deborah T. Hung b, Zhiguo Zhou a a Luna Innovations Inc., Danville, VA 24541 b The Broad Institute, Cambridge, MA 02142; Departments Molecular Biology Medicine, Massachusetts General Hospital, Boston, MA 02114; Email: xuy@luaninc.com, Tel: 434-483-4232 Bacterial infections have become the major threats to human communities worldwide. Rapid, accurate identification of the etiologic bacteria is critical for guiding effective antimicrobial therapy and improving patient outcomes, and for reducing the length of hospitalization and associated costs. Clinical bacteriology has traditionally relied on phenotypic methods to identify bacterial pathogens and determine antimicrobial susceptibility. These are relatively inexpensive but have a turnaround time of several days because of the time needed for bacterial growth and the sequential nature of sample processing. The identification turnaround time has been reduced by the introduction of automated instruments, however, these techniques continue to rely for the large part on bacterial growth and take long time for sample preparation, so there is an urgent need to develop new methods to speed up the sample preparation. So far, there is no currently commercial products for low cost broad spectrum bacterial collection in a very short period of time (< 0.07%) toward blood cells, proteins and other molecules present in human blood. Importantly, the magnetically purified and separated bacteria samples were successfully identified by both the traditional culturing method and also by an exemplary advanced RNA diagnostic platform NanoString’s nCounter System.