Binding Studies of Histamine-imprinted Polymers Prepared using Photochemical Polymerization for facile incorporation to sensing device

E.F. Romano, R.C. So, C. Holdsworth
Ateneo de Manila University,

Keywords: histamine sensing, photopolymerization, processable polymers


Accurate determination of histamine (HTM) levels in food or in samples from biological origin (e.g., tissues, urine) is important for monitoring HTM-related food poisoning or pathophysiological conditions. In this study, the imprinted polymers were accessed using photochemical polymerization using 4% monomer containing 80 or 90 wt% EGDMA and 20 or 10 wt% MAA, and histamine:MAA mole ratio of 1:4 in acetonitrile. Four polymers with sizes ranging from 197 to 57 nm in the collapsed state were evaluated: histamine-imprinted polymers (PCP-M80 with 80% EDGMA, and PCP-M90 with 90% EDGMA) and the corresponding non-imprinted polymers (PCP-N80, PCP-N90). Binding studies using frontal analysis capillary electrophoresis (FACE) showed that the binding capacity of the imprinted polymers were higher than the non-imprinted counterpart, indicative of imprinting effect. PCP-M80 was also found to have more binding sites than PCP-M90. However, PCP-M90 has higer concentrations of high-affinity sites. These results indicate that PCP-M80 to be more selective to histamine compared to the other polymers studied. It is also anticipated that this strategy will allow convenient grafting of the polymers onto activated substrates for subsequent sensor preparation.