Affinity Sensor based on Immobilized Molecular Imprinted Synthetic Ligands

P. Lenain, B. Mattiasson, M. Hedström, S. De Saeger
Ghent University, BE

Keywords: submersible, capacitive, molecularly imprinted polymers, performs in river

Summary:

A sensing unit which can be submerged in a river to monitor and continuously sample selected substances of interest should be developed. Moreover, it should operate independently over a longer period of time, e.g. several weeks or months. Therefore we have developed an affinity sensor based on capacitive transduction, which would be able to perform as sensing device in such unit. Molecularly imprinted polymers towards a model compound, metergoline, were developed and used as recognition element. Thus, a robust sensor was obtained which regenerated spontaneously, and to a full extent, under the conditions naturally imposed in a river. Metergoline could be measured in water without manipulation of the binding or regeneration conditions, thereby offering a viable tool for on-site application. The limit of detection was 1 µM and a working range from 1 - 50 µM, the maximum solubility in aqueous solvent, was established.