Effect of Coating Materials on Cellular Uptake of Microparticles in HeLa Cells

N. Kato, A. Kojima
Meiji university, JP

Keywords: cellular uptake, microparticles

Summary:

We investigated the effect of coating materials on cellular uptake using the polyelectrolyte-coated particles. The negatively charged fluorescent latex (1μm in diameter, emission at 483nm) was coated using the polycations; polyethylenimine (PEI), polyallylamine hydrochloride (PAH), poly-L-lysine hydrochloride (PLL), and poly-L-arginine hydrochloride (PLA). The uptake and adhered amounts of the particles were counted from the 3D images of the cell obtained by nonlinear optical microscope. Our observation revealed that the particle adhesion to the cell is govern by the surface charge of the particle and not by the material, but the cells distinguish the materials when they uptake and they favor uptaking the particles coated by the cationic polypeptides (PLL and PLA) rather than the synthetic polycations (PEI and PAH).