Multifunctional Nanoparticles for PET,MRI, and Long Wavelength Fluorescence Imaging

C. Tang, N.M. Pinkerton, D. Gao, S. Li, B.R. Benson, J. Anthony, P.J. Sinko, R.K. Prud’homme
Princeton University, US

Keywords: imaging, nanoparticle, MRI, PET, fluorescence, therognostic

Summary:

We describe a nanoparticle assembly process to produce multifunctional nanoparticles (NPs) in a single step. This is in contrast to complicated multistep assembly processes previously described in the literature. The rapid precipitation process (Flash NanoPrecipitation: FNP) enable encapsulation of SPIO particles long wavelength fluorphores, and therapeutic agents in the same NP core, with the NPs having a protective PEG steric layer. The PEG layer produces circulation time over two days. Using OH terminated PEG chains, targeting to the liver in vivo is demonstrated by both MRI and long wavelength fluorescence. The particles can also incorporate PET agents through an iodination reaction on a poly-phenol encapsulated in the NP core, or through Zr chelated to DFO chelators on the surfaces of the nanoparticles.