Thymoquinone nanoparticle formulation and in vitro efficacy

I.H. Fakhoury, W.S. Saad, H.U. Gali-Muhtasib, R. Schneider-Stock
American University of Beirut, LB

Keywords: thymoquinone nanoparticle flash nanoprecipitation

Summary:

The work summarized here pertains to the formulation of thymoquinone into controlled size nanoparticles using a recently developed solvent precipiation technique termed Flash Nanoprecipiation. Using a diblock copolymer of poly(styrene-b-ethylene oxide) as the stabilizing polymer, nanoparticles with a high thymoquinone loading were formulated by Flash Nanoprecipitation. The resulting nanoparticle size was 78 nm as determined by dynamic light scattering. The in vitro activity of thymoquinone in the nanoparticle formulation as well as its free form were evaluated against MCF-7 and MDA-MB-231 breast cancer cell lines. The results demonstrate enhanced antitumor activity of the thymoquinone nanoparticle formulation compared with the free form after 72 hours.