Detection of Prostate-Specific Antigen (PSA) with an Ultra High Performance Electrode Modified with Sol-Gel Au Nanoparticles

S.W. Leung, S. Mozneb, J.C.K. Lai, D. Assan
Idaho State University, US

Keywords: PSA, prostate cancer, high performance electrode, nanogold particles, biosensor

Summary:

Prostate cancer is one of the leading cancer causing death in men, especially in western developed countries. Prostate-specific antigen (PSA) is one of the biomarker that can be used to diagnose prostate cancer, although using PSA as the sole diagnosis tool for prostate cancer remains a debatable issue. Numerous attempts have been made to find the utmost acceptable method to detect prostate cancer. Among those, electrochemical method is deemed most sensitive in detecting biomarkers, and PSA is the common-most biomarker used for cancer detection. Our research group is in the quest of developing a biosensor platform that is fast in response time and versatile that can be used to detect a variety of chemicals species and metabolites. In this study, we have modified a gold electrode with layers of sol-gel nanogold particle composite that could detect PSA concentrations below 10 fg/ml in aqueous solutions, that is at least an order of magnitude more superior than the literature reports. The sensitivity range and stability of the biosensor were investigated. This sensor platform can be adapted to simultaneously detecting multiple biomarkers which is believed to be the more sensible approach for the cancer detection.