α-Lactalbumin nanoparticles for delivery of bioactive compounds: Preparation, characterization and in vitro evaluation

S. Uzun, J.L. Kokini
University of Illinois, US

Keywords: α-lactalbumin nanoparticles, encapsulation, nanoprecipitation, milk protein

Summary:

Biodegradable nanoparticles produced from α-lactalbumin were developed as novel carriers for drug delivery. Nanoprecipitation technique is widely used to prepare nanoparticles for drug delivery. α-Lactalbumin nanoparticles were synthesized by employing this simple method. Nanoparticles were generated by dropwise addition of ethanol into protein solution. The particles were characterized by Dynamic Light Scattering and morphology of particles was observed by Scanning Electron Microscopy. The influence of ethanol and lactalbumin concentration on particle size was studied in order to obtain smaller nanoparticles. With using this method, nanoparticles less than 200 nm were produced.The zeta potential value of nanoparticles in DI water was found -18.3 ± 2.1 mV. The yield of nanoparticulation was assessed using Bradford assay. The isoelectric point of α-lactalbumin nanoparticles was determined by carrying out the zeta potential measurement to α-lactalbumin nanoparticles dispersed in buffer solution at different pH values. Curcumin was used as a model drug and entrapped into protein nanoparticles during precipitation. Encapsulation efficacy was calculated and in vitro release study of encapsulated nanoparticles were performed by measuring the curcumin release for 72h.