Hyperspectral Imaging System as a Screening Tool for Nanomaterial Identification and Characterization

S. Tahiliani, G. Roth, N. Neu-Baker, S. Brenner

Keywords: dark-field microscopy, hyperspectral imaging, materials analysis, nanotechnology, nanomaterials, screening, spectral library, spectral mapping, spectrophotometry, metrology


Submitted for your consideration is a research work entitled “Hyperspectral Imaging System as a Screening Tool for Nanomaterial Identification and Characterization” which provides a view of new and emerging applications of hyperspectral imaging at the nanoscale. Hyperspectral microscopy is an advanced visualization technique that combines hyperspectral imaging with state-of-the-art optics and computer software to enable the rapid identification of various materials at the micro- and nanoscales. This topic is of broad interest to the scientific community, as nanoscale imaging applications have applicability in a diverse array of research fields spanning many disciplines. A critical challenge faced by researchers, scientists, and developers is that of locating, identifying, and characterizing nanomaterials in a variety of matrices – a challenge that this new tool is poised to tackle. This work serves to describe the science of hyperspectral microscopy and how Brenner research group has used it to serve as a screening tool for nanomaterials in biological samples.We have used the HSI system to analyze rat lung, lung-associated lymph tissue, liver, kidney, and spleen tissues following inhalation exposures to metal oxide nanoparticles in order to characterize the deposition of nanoparticles in the primary (lung) tissue and translocation to secondary (lymph, liver, kidney, spleen) tissues.