A novel and facile approach for in situ synthesis of silver nanoparticles in nanofibers for antimicrobial wound dressing

P. Dubey, P. Gopinath
Indian Institute of Technology Roorkee, IN

Keywords: nanofibers, silver nanoparticles, wound dressing, antimicrobial

Summary:

The purpose of the present study was to develop stable and porous PEO-PCL (Polyethylene glycol)-(Polycaprolactone) blended silver nanoparticle composite nanofiber scaffolds for chronic wound healing applications. A novel and simple approach for in situ synthesis of silver nanoparticle was explored without use of any external reducing, stabilizing and cross-linking agent. In this synthesis method dimethyl formamide (DMF) along with dicholormethane (DCM) was used as solvent where DMF also act as reducing agent for silver nanoparticle formation. Stabilization of nanoparticle in fiber was accomplished by the presence of high molecular weight PEO polymer which in turn acts as reducing agent along with DMF for silver nanoparticle formation. The successful synthesis of crystalline silver nanoparticle was done and which was confirmed by UV-visible spectroscopy, and Transmission electron microscopy (TEM) analysis. TEM has shown small size (16-17 nm) nanoparticle formation. Field emission scanning electron microscopy (FE-SEM) images showed formation of porous and smooth nanofibers. PEO-PCL blended nanofiber alone was 300-370 nm in diameter but presence of silver nanoparticle further decreases their diameter to 200-250 nm. The nanofibers synthesized by above approach can be used as future nanomedicine for wound dressing against wide range of bacterial community.