Non Toxic Water Soluble Nano Carbon Particles that can pass through the “Blood Brain Barrier”

A. Allam, S. Sarkar, M. Ghosh, B. Pakhira
Cromoz Inc, US

Keywords: carbon dots, blood brain barrier, drug delivery

Summary:

The tight junctions between endothelial cells are known to be responsible for the brain blood barrier (BBB) function. Significant medical research is being done in this field to find particles that can pass this barrier. The fluorescent, non-toxic, water-soluble spherical nano carbon particles with structure similar to a layered onion (carbon onions) have been synthesized by us . We tested these on the anesthetized mouse after artificial intubation. It was subjected to a chronic cranial window to get a baseline using fluorescence intravital microscopy . The images were captured before and after the intravenous injection of nano carbon onion. We observed that the nano carbon particles were able to pass through the blood brain barrier (BBB) and had entered the brain without causing any perfusion. This in-vivo study clearly shows where normal dye cannot pass the BBB, our nano carbon particles (carbon onions) have successfully crossed this BBB. This raises immense possibilities for drug delivery in the brain. Further, we have also conjugated these non-cytotoxic carbon nano particles with anti-cancer drug such as paclitaxol. Our research has shown that such conjugated drug can be released slowly in a sustained manner.