Antitumor efficacy of niclosamide loaded PEO:PEI fibers with tunable release profile

S. Uday Kumar, P. Gopinath
Indian Institute of Technology Roorkee, IN

Keywords: nanofibers, niclosamide, drug delivery, apoptosis, cancer therapy

Summary:

In the recent past, there has been rapid surge in reports indicating the anti-neoplastic potential of a drug called niclosamide. The hydrophobic nature of niclosamide limits its in vivo application. A suitable carrier for its controlled and sustained release is yet to be formalized. In this work, a biocompatible poly (ethylene oxide) (PEO) and polyethylenimine (PEI) based polymeric nanofibers has been fabricated by electrospinning for encapsulation and delivery of niclosamide. The as-synthesized fibers were characterized by field emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM). Thermogravimetric analysis (TGA) and X-ray diffraction (XRD) analysis were performed to confirm the physiochemical status of the drug incorporated in the fibers. The drug release study was carried out in a buffered release medium under static conditions for 72 hours. A sustained and controlled release profile was observed. The drug loaded fibers antitumor efficacy was validated against A549 cells by cell viability assay (MTT assay) and by nuclear staining. The treated cells were also subjected to gene expression studies to elucidate the mechanism of drug action. Thus, this study clearly outlines the therapeutic potential of a novel anticancer drug niclosamide, and also validates a suitable drug delivery platform for the same.