Silver nanospheres and silver nanotriangles reducing Candida albicans in dental devices

G. Hernàndez-Padròn, T. Gòmez-Quintero, L.M. Lòpez-Marìn, V. Castaño, M.C. Arenas, J. de la Fuente-Hernàndez, L.S. Acosta-Torres
Centro de Fìsica Aplicada y Tecnologìa Avanzada, UNAM, MX

Keywords: UV-Vis spectroscopy, electron transmission microscopy, biocompatibility, in vitro, Candida albicans

Summary:

In Dentistry, it is important to reduce the Candida albicans adherence in dental prosthetic devices; in this sense, the fungicidal effect of silver nanoparticles is continuously studied. The present study is about the fungicidal activity of silver nanoparticles, in the shapes of nanospheres and nanotriangles, obtained through two different reducing agents (geranium leaves and sodium borohydride), on Candida albicans cultures. The nanoparticles in both morphologies were incorporated in a matrix of poly(methyl-methactylate) [PMMA]. The adherence of Candida albicans were evaluated on the acrylics samples; after that, the cytotoxicity assay and the genotoxicity assay were carried out for all experimental groups. The commercial acrylic (Nature Cryl) and the single PMMA were the control groups. One-Way ANOVA analysis showed that the PMMA with spherical nanoparticles obtained using the borohydride reagent and the PMMA with triangle nanoparticles showed higher Candida albicans adherence (p<0.05). The PMMA with spherical nanoparticles obtained with geranium presented lower adherence of the fungus (p=0.01); while in the cell viability assays (cytotoxicity and genotoxicity) there were no significant difference (p>0.05) between all the tested groups. The use of geranium is the best option to synthesize spherical silver nanoparticles, which added into PMMA result in lower Candida adherence maintaining the biocompatibility.