Unique Volatolomic Signature of P53 and KRAS Oncogenes in Lung Cells

O. Barash
Technion- Israel Istitute of Technology, IL

Keywords: lung cancer, nanoarray, volatile

Summary:

Gene profiling became a key role for accurately classifying tumors in individual patients, for predicting the response to the available treatments and for personalizing cancer treatment. Here, we show the ability to detect lung cancer related oncogenes through volatolomic signature. Human bronchial epithelial cells (HBEC) cell-lines carrying the K-RasV12 mutation, knock-down of p53 or both were compared with parental HBEC cells and with each other. The method is based on the detection and identification of patterns of volatile organic compounds (VOCs) emitted from the cell membranes, using a tailor-made nanoarray of gold nanoparticles sensors combined with pattern recognition methods (e.g. DFA). The sensor array is composed of cross-reactive nanosensors which respond to the collective VOCs. Thus in combination with DFA a unique volotalomic signature is designed for each mutation. While the reported concept was obtained through in vitro studies, as a way to eliminate confounding factors that are associated with clinical samples or patients/animals, it is reasonable to assume that similar findings could be detected directly from blood or the exhaled breath samples which will allow immediate prediction for targeted therapy.