PEGylated Gold Nanorods: A development for the separation and characterization of biocompatible nanoparticles

T.M. Nguyen, J.C. Gigault, V.A. Hackley
National Institute of Science and Technology, US

Keywords: nanometrology, field flow fractionation, PEG, gold nanorods

Summary:

Asymmetric-flow field flow fractionation (A4F) is utilized as an elution based separation technique employed for the characterization of gold nanorods (GNR). For the successful continual development of biological applications of GNRs, knowledge of the aspect ratio (AR) distribution of GNRs modified with polyethylene glycol (PEG) and its chain structure is important. GNR of varying AR were obtained both commercially and synthesized using template methods. The nanorods are functionalized with PEG having different molar masses. The development of an A4F separation and characterization methodology with MALS, DLS, and UV-Vis detection for PEGylated GNR in a non-cytotoxic mobile phase is demonstrated. These new A4F results identically follow the results from a previously established methodology by Gigault et al. in which, the parameter that principally controls elution of GNRs is their aspect ratios. The developed methodology applied to biocompatible GNRs can be utilized in the future for purification and characterization of GNR for in vivo AR specific therapy and treatments.