Nanogenotoxicity Measurements of Oxidative DNA Damage Using Isotope Dilution Mass Spectrometry

E. Petersen, P. Jaruga, M. Dizdaroglu, B. Nelson
National Institute of Standards and Technology, US

Keywords: nanogentoxoicity, nanoparticles, nano-EH&S, mass spectrometry

Summary:

Hyphenated mass spectrometry (gas chromatography/mass spectrometry) techniques have been employed as one of the primary analytical tools for investigating the effects of ionizing radiation, chemical/biological carcinogens and oxygen derived free radicals on the induction and subsequent repair of oxidatively-induced DNA damage (DNA lesions) in living systems. Certain DNA lesions, such as 7,8-dihydro-8-oxoguanine (8-OH-Gua), are not only established mutagens, but have also been utilized as biomarkers of systemic oxidative stress. The National Institute of Standards and Technology (NIST) has established a comprehensive research program focused on identifying and characterizing the DNA damaging mechanisms of commercially relevant engineered engineered nanoparticles (NPs) using models of increasing biological complexity through the application of isotope-dilution hyphenated mass spectrometry tools for the quantification of oxidatively-induced DNA damage. We present an overview of our recent findings from studies on metal (AuNP and AgNP), metal oxide (CuO NP), and carbon-based (SWCNTs and MWCNTs) nanoparticles.