Investigating Modified Oligonucleotides as Robust, Programmable Release Agents

V. Tohver Milam, N. Eze
Georgia Institute of Technology, US

Keywords: locked nucleic acids, programmable release, colloidal particles, flow cytometry

Summary:

While LNA has been examined as a therapeutic agent in various antisense scenarios, we are exploring LNA as a programmable biomaterials assembly and disassembly tool under isothermal conditions. To drive isothermal disassembly of LNA-linked objects, we employ displacement strategies in which one hybridization partner is replaced by another. While LNA shows great promise in these schemes, a clear understanding of its hybridization activity is lacking in the literature. In the current study, we use flow cytometry to compare the in situ displacement activity of LNA and DNA-based targets on microsphere surfaces. By monitoring displacement events as they occur on microsphere surfaces, we can directly assess and compare the competitive abilities of natural and modified oligonucleotides in the absence of any experimental artifacts arising from conventional wash steps prior to analysis.